As a chemical biology group we have the overarching goal to extend the power of synthetic chemistry to complex biological systems. One of our major interests is the development of methods for the selective labeling of biomolecules (e.g. proteins) with small molecule probes inside living cells and its application for biophysical methods. Design and synthesis of probes and markers, is an essential part of our work to provide tailor made molecules for advanced biophysical methods to study specific biological questions. The long term goal of this research is to develop methods and probes that allow dynamic biological processes to be observed and/or manipulated in living cells. Just as biophysical methods for studying biomolecules in vitro have significantly impacted our fundamental understanding of biomolecule structure and function, the ability to image protein networks in living cells has the potential to make broad, significant contributions to our understanding of the mechanism of biological pathways and human disease.
Current research topics of the lab:
· Selective protein labeling in complex biological systems (living cells, in vivo)
· Development and application of bio-orthogonal chemistry
· Development and application of biophysical probes
(single molecule imaging, super-resolution microscopy, molecular and metabolic imaging)
· Protein/Enzyme regulation by using light as an external trigger